| 万家颖
，王银.基于褪黑素的分子探针在胶质母细胞瘤成像中的研究[J].神经损伤功能重建,2023,(2):77-79 |
| 基于褪黑素的分子探针在胶质母细胞瘤成像中的研究 |
| Application of A Melatonin-Based Molecular Probe for Glioblastoma Imaging |
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| DOI： |
| 中文关键词: 褪黑素 分子探针 成像 胶质母细胞瘤 |
| 英文关键词: melatonin molecular probe imaging glioblastoma |
| 基金项目: |
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| 摘要点击次数: 1110 |
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| 中文摘要: |
| 目的：探讨一种基于褪黑素（MT）的分子探针对胶质母细胞瘤的成像效果。方法：以5-甲氧基色胺（褪
黑素）为原料，将其与荧光染料Cy5偶联制得探针Cy5-MT。采用MTT法测试探针Cy5-MT对胶质母细胞瘤
细胞U87和正常的人脑胶质细胞HEB的细胞毒性；采用流式细胞实验测试探针Cy5-MT对胶质母细胞瘤细
胞U87的靶向性；采用激光共聚焦实验测试探针Cy5-MT对U87的成像效果。结果：探针Cy5-MT的结构通
过质谱进行确证。Cy5-MT对 U87和HEB的毒性较小，Cy5-MT在U87细胞中的百分摄取率显著高于HEB
细胞；Cy5-MT能够特异性地对U87细胞进行成像。结论：探针Cy5-MT能够特异性的靶向胶质母细胞瘤U87
细胞，实现对U87的精准成像，且生物安全性高。 |
| 英文摘要: |
| To explore the imaging effect of a melatonin-based molecular probe in glioblastoma.
Methods: The probe Cy5-MT was prepared by coupling 5-methoxytryptamine with fluorescent dye Cy5. The in
vitro MTT assay was used to evaluate the toxic effect of Cy5-MT on glioblastoma cell U87 and normal human glial cell HEB; Flow cytometry was used to evaluate the targeting ability of Cy5-MT to U87 and HEB; laser scanning confocal microscopy was used to evaluate the imaging effect of Cy5-MT on U87. Results: The structure of
Cy5-MT was confirmed by mass spectrometry. In vitro cytotoxicity study showed that Cy5-MT had low toxicity
to U87 and HEB, indicating high biosafety. Flow cytometry results showed that the percentage of cellular uptake
and mean fluorescence intensity of the probe per U87 cells were significantly higher than the normal HEB cells.
Imaging results showed that Cy5-MT can accurately image U87 tumor cells. Conclusion: The probe Cy5-MT
can specifically target U87 glioblastoma cells to achieve accurate imaging of U87 and also demonstrates high biosafety. |
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