| 丁君
，郑瑾
，范威.HIF-1α和Notch1在原代培养的神经元及胶质细胞的混合体系中的相互作用[J].神经损伤功能重建,2021,16(12):695-697 |
| HIF-1α和Notch1在原代培养的神经元及胶质细胞的混合体系中的相互作用 |
| Interaction between HIF-1α and Notch1 in a Mixed System of Primary Cultured Neurons andGlial Cells |
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| DOI： |
| 中文关键词: 神经元 胶质细胞 缺氧诱导因子-1 Notch1 |
| 英文关键词: neurons glial cells HIF-1α Notch1 |
| 基金项目: |
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| 中文摘要: |
| 目的：观察在原代混合培养的神经元及胶质细胞的体系中，HIF-1α和Notch1基因间是否存在相互作
用。方法：构建体外原代培养神经元及胶质细胞的混合体系，应用电穿孔转染方法分别沉默 HIF-1α和
Notch1基因。RT-PCR检测HIF-1α和Notch1基因转录水平，Western blot方法检测HIF-1α和Notch1蛋白表
达水平。结果：沉默HIF-1α基因后，HIF-1α和Notch1基因转录及蛋白表达水平均显著下降；沉默Notch1基
因后，HIF-1α和Notch1基因转录及蛋白表达水平均显著下降。结论：在原代培养的神经元及胶质细胞的混
合体系中，HIF-1α和Notch1之间可能存在相互作用的关系。 |
| 英文摘要: |
| To investigate whether there is an interaction between HIF-1α and Notch1 in co-cultured
neurons and glial cells. Methods: Cortical neurons and glial cells were co-cultured in vitro. The HIF-1α and
Notch1 genes in the co-cultured cells were muted by electroporation transfection, then the transcription levels
and protein expression levels of Notch1 and HIF-1α were detected by RT-PCR and Western Blot, respectively.
Results: After silencing either the HIF-1α or Notch1 gene, the transcription and protein expression levels of
both HIF-1α and Notch1 were reduced. Conclusion: There may be an interaction between HIF-1α and Notch1
in the neuron and glial cell co-culture system |
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