|
| 原位递送槲皮素的PECT温敏水凝胶对大鼠脊髓损伤的修复作用 |
| Repair efficacy of PECT thermosensitive hydrogel for in situ delivery of quercetin in rat spinal cord injury |
| 投稿时间：2024-04-08 修订日期：2024-04-08 |
| DOI： |
| 中文关键词: 脊髓损伤 槲皮素 QR-PECT水凝胶 氧化性 |
| 英文关键词: spinal cord injury quercetin QR-PECT hydrogel oxidative properties |
| 基金项目:中央级公益性科研院所基本科研业务费专项资金项目（2021CZ-9） |
|
| 摘要点击次数: 70 |
| 全文下载次数: 0 |
| 中文摘要: |
| 【摘要】目的：探讨负载槲皮素（QR）的含环醚侧基聚己内酯-聚乙二醇三嵌段共聚物（PECT）温敏水凝胶对大鼠脊髓损伤（SCI）的修复作用。方法：制备负载槲皮素且37℃条件下自动成胶的温敏水凝胶（QR-PECT）；表征水凝胶的温敏及流变特性；通过CCK-8实验评价水凝胶的细胞相容性；通过1,1-二苯基-2-吡啶并肼基（DPPH）自由基清除实验来评估水凝胶清除自由基的能力；通过活性氧（ROS）检测试剂盒评价水凝胶清除细胞内ROS的能力；制备大鼠脊髓损伤全切模型，分为SCI组、QR组及QR-PECT水凝胶组，应用BBB评分评估大鼠运动功能的恢复，应用尼氏染色观察神经元迁移浸润情况，通过H&E染色评价水凝胶生物相容性。结果：制备的QR-PECT聚合物在37℃条件下形成水凝胶，并持续释放槲皮素；CCK-8实验定量结果显示水凝胶与对照组的细胞活力相当；DPPH清除实验显示，水凝胶的DPPH清除率达到60%，与QR组相当；通过测量HT22细胞内的ROS荧光值，发现水凝胶显著降低了HT22细胞内荧光强度；脊髓损伤后8周，水凝胶组大鼠运动功能明显恢复，尼氏染色显示水凝胶组存在大量神经元浸润，H&E染色结果显示水凝胶组心、肝、脾、肺、肾与正常大鼠没有明显的差异。结论：QR-PECT水凝胶具有良好的生物相容性和抗氧化性，并能够促进大鼠脊髓损伤后神经元的再生和运动功能恢复。 |
| 英文摘要: |
| 【Abstract】Objective: To investigate the reparative effects of polycaprolactone-polyglycol triblock (PECT) thermosensitive hydrogel loaded with quercetin on spinal cord injury (SCI) in rats. Methods: The temperature-sensitive hydrogel (QR-PECT) was prepared to release quercetin at 37℃; The temperature sensitive and rheological properties of the hydrogel were characterized; Cell compatibility was assessed using CCK-8; Free radical scavenging ability was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH); Cellular reactive oxygen species (ROS) levels were measured to assess ROS removal capacity; A rat model of spinal cord injury (SCI) was established and divided into three groups: the SCI group , the QR group and QR-PECT hydrogel group; Motor function recovery in rats was evaluated, neuronal migration and infiltration were observed by Nissl staining, and biocompatibility of the hydrogel was assessed by H&E staining. Results: The QR-PECT hydrogel exhibited sustained release of quercetin at 37℃; cell viability quantified by CCK-8 assay demonstrated comparable results to the control group; DPPH clearance assay revealed a clearance rate of 60%, equivalent to the QR group; Measurement of ROS fluorescence intensity in HT22 cells indicated significant reduction after treatment with the hydrogel; At eight weeks post-injury, significant motor function recovery was observed in the hydrogel group along with massive neuronal infiltration confirmed by Nissl staining. No significant differences were found between heart, liver, spleen, lung, kidney samples from normal or treated rats based on H&E staining. Conclusion: The QR-PECT hydrogels demonstrate exceptional biocompatibility and antioxidant characteristics, thereby promoting neuronal regeneration and enhancing motor function. |
|
View Fulltext
查看/发表评论 下载PDF阅读器 |
| 关闭 |