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| LncRNA MIRLET7BHG对人神经细胞SH-SY5Y氧糖剥夺再灌注损伤的保护作用研究 |
| Protective Effect of LncRNA MIRLET7BHG on SH-SY5Y Cells after Oxygen and Glucose Deprivation Reperfusion InjuryXu Chen-guang,Li Jing-jing,Wang Liang,He Li. |
| 投稿时间：2023-10-24 修订日期：2023-10-24 |
| DOI： |
| 中文关键词: LncRNA LncRNA MIRLET7BHG 缺血再灌注 神经细胞 凋亡 |
| 英文关键词: LncRNA LncRNA MIRLET7BHG Ischemia/reperfusion Neuronal cells Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的：旨在探究LncRNA MIRLET7BHG在人神经细胞SH-SY5Y缺血再灌注(I/R)损伤中的作用。方法：采用氧糖剥夺/复氧(OGD/R)模型诱导体外SH-SY5Y细胞I/R损伤,并通过RT-qPCR检查LncRNA MIRLET7BHG的表达水平。构建SH-SY5Y 细胞LncRNA MIRLET7BHG敲低模型,利用MTT、流式细胞术和蛋白质印迹法评估常规培养或OGD/R处理后细胞的活力和凋亡水平。结果：OGD/R处理后,SH-SY5Y细胞中的LncRNA MIRLET7BHG表达显著增加。敲低LncRNA MIRLET7BHG抑制了常规培养或OGD/R处理的SH-SY5Y的活力,促进了细胞凋亡。结论：LncRNA MIRLET7BHG在氧糖剥夺/复氧诱导的SH-SY5Y细胞凋亡中有一定的抑制作用,而上调的LncRNA MIRLET7BHG表达水平,这可能表明存在某种负反馈机制。这一发现为进一步理解该LncRNA在神经保护机制中的作用提供了重要线索,有望为神经系统疾病的治疗提供新的治疗策略。 |
| 英文摘要: |
| Objective: The aim of this study was to investigate the role of LncRNA MIRLET7BHG in ischemia/reperfusion (I/R) injury in human neural cells SH-SY5Y. Methods: The OGD/R model was used to induce I/R injury in SH-SY5Y cells in vitro, and the expression levels of LncRNA MIRLET7BHG were examined using RT-qPCR. A knockdown model of LncRNA MIRLET7BHG was established in SH-SY5Y cells, and the viability and apoptosis levels of cells under regular culture or OGD/R treatment were assessed using MTT assay, flow cytometry, and Western blotting. Results: After OGD/R treatment, the expression of LncRNA MIRLET7BHG significantly increased in SH-SY5Y cells. Knockdow n of LncRNA MIRLET7BHG inhibited the viability of SH-SY5Y cells under regular culture or OGD/R treatment and promoted cell apoptosis. Conclusion: LncRNA MIRLET7BHG has a certain inhibitory role in oxygen sugar deprivation/reoxygenation-induced apoptosis in SH-SY5Y cells Neurological injury plays a protective role in the up-regulated expression level of LncRNA MIRLET7BHG, which may indicate the existence of some kind of negative feedback mechanism. This finding provides important clues for further understanding the role of this LncRNA in neuroprotective mechanisms and holds promise for providing new therapeutic strategies for neurodegenerative diseases. |
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