文章摘要
宁珑，孙航，周艳凯，冯晋，许春萍，何芳雁.线栓法复制大鼠脑缺血后出血转化模型的研究[J].神经损伤功能重建,2022,17(知网首发):
线栓法复制大鼠脑缺血后出血转化模型的研究
Study on Rat model of Hemorrhagic Transformation after Cerebral Ischemia by SutureOcclusion Method
  
DOI：
中文关键词: 脑缺血后出血转化模型  线栓法  再灌注
英文关键词: hemorrhagic transformation after cerebral ischemia model  suture method  reperfusion
基金项目:云南省科技厅科技 计划项目中医联合 重点项目（No. 017 FF117-004）； 云南省科技厅科技 计划项目基础研究 专项（No. 202001A T070138）； 国家自然科学基金 资助项目（No. 815 60664)； 云南省科技厅科技 计划项目中医联合 青年项目（No. 201 8FF001-074）； 云南省科技厅基础 研究专项-面上项 目（No. 202001AT0 70554）
作者单位
宁珑，孙航，周艳凯，冯晋，许春萍，何芳雁 云南中医药大学中 药学院 
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中文摘要:
      目的：探讨线栓法复制大鼠脑缺血后出血转化模型的最佳条件。方法：采用线栓法复制大鼠大脑中 动脉阻塞模型（tMCAO），按脑血流变化情况及术后2 h的神经学评分结果选择造模成功大鼠并再随机分为 3组，分别在在脑缺血3 h、6 h和9 h拔出线栓恢复脑血供，复制大鼠脑缺血后出血转化模型（tMCAO-HT）。 术后48 h以脑组织梗死情况、HT发生率、脑组织出血量评分、脑组织血红蛋白含量以及死亡率为指标，评估 筛选最佳的tMCAO-HT模型复制条件。结果：与假手术相比，各模型组脑组织缺血后5 min测得脑血流量 均下降至基础值的42%～53%，再灌注后5 min测得脑血流量均显著提高；术后2 h神经学评分均明显升高 （P＜0.01），且组间无明显统计学差异；术后48 h各模型组脑梗死灶明显，且均出现不同程度的HT；HT的出 血量评分和血红蛋白含量均明显高于假手术组（P＜0.05或P＜0.01），且随着缺血时间的延长呈逐渐上升趋 势；各模型组均有不同程度的大鼠死亡，且随着缺血时间的延长，大鼠死亡率呈增加趋势，其中缺血9 h组死 亡率高达 83.33%。结论：线栓法造模导致脑缺血后 3 h、6 h、9 h 再予以再灌注均能成功复制大鼠 tMCAO-HT模型，但缺血9 h导致死亡率过高，研究用tMCAO-HT模型以脑缺血3 h或6 h后再灌注为宜。
英文摘要:
      To explore optimal conditions for the suture occlusion method to make the model of hemorrhage transformation after cerebral ischemia in rats. Methods: The rat model of transient middle cerebral artery occlusion (tMCAO) was established by the suture occlusion method. According to the changes in cerebral blood flow and results of neurological assessment 2 hours after operation, the rats that underwent successful model establishment were selected and randomly divided into 3 groups. The suture was removed at 3, 6, and 9 hours after cerebral ischemia to make hemorrhage transformation after cerebral ischemia (tMCAO-hemorrhagic transformation, tMCAO-HT). The cerebral infarction, incidence of HT, cerebral hemorrhage volume, hemoglobin content of brain tissue and mortality rate were used as indicators at 48 hours postoperatively to screen for optimal tMCAO-HT rat model replication conditions. Results: Compared with the sham operation group, the cerebral blood flow in each model group measured 5 minutes after cerebral ischemia decreased to 42%～53% of the baseline value; when measured 5 minutes after reperfusion, the blood flow in each model group was significantly increased. The neurological scores at 2 hours after operation were significantly increased (P<0.01), and there was no significant statistical difference between the groups. There was obvious cerebral infarction and HT in various degrees in each model group 48 hours after operation. The HT hemorrhage volume and hemoglobin content in each model group were significantly greater than those of the sham operation group (P<0.05 or P<0.01), and these values gradually increased with the prolongation of ischemia. Each model group showed different degrees of mortality, and mortality rates increased with the increased ischemia time, in which the mortality rate of the 9-hour ischemia group reached 83.33%. Conclusion: The tMCAO-HT model in rats can be successfully replicated by reperfusion at 3, 6, and 9 hours after cerebral ischemia with the suture method. However, the mortality rate at 9 hours is too high, and reperfusion after 3 or 6 hours of cerebral ischemia is more appropriate in studies using the tMCAO-HT model.
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