| 林琅，黎红华，骆文静，吴非.丁苯酞对慢性缺血性脑白质损害的保护作用研究[J].神经损伤功能重建,2022,17(知网首发): |
| 丁苯酞对慢性缺血性脑白质损害的保护作用研究 |
| Protective Effect of Butylphthalide on White Matter Damage in Chronic Cerebral Hypoperfu⁃sion |
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| DOI： |
| 中文关键词: 慢性低灌注 脑白质损害 血脑屏障 微循环 丁苯酞 |
| 英文关键词: chronic cerebral hypoperfusion white matter impairment blood brain barrier microcirculation butylphthalide |
| 基金项目:湖北省卫生健康
科 研 联 合 项 目
（No. WJ2019H1
18） |
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| 中文摘要: |
| 目的：观察丁苯酞可否改善慢性低灌注所致脑白质损害并探讨其可能的机制。 方法：72只大鼠随机分
为对照组、低灌注组及丁苯酞组，每组24只。低灌注组及丁苯酞组通过阻断双侧颈总动脉血流建立慢性前循
环低灌注模型，丁苯酞组于术后给予丁苯酞注射液腹腔注射。建模30 d后，通过荧光素异硫氰酸酯-葡聚糖静
脉注射结合共聚焦激光扫描法检测脑微血管密度，通过伊文思蓝静脉注射后测定脑组织荧光强度检测血脑
屏障完整性，通过Kluver-Barrera氏髓鞘染色检测白质纤维密度。结果：丁苯酞组大鼠脑白质微血管密度高于
其他2组（均P＜0.05）。低灌注组大鼠伊文思蓝浓度较对照组大鼠显著增高（P＜0.01）；丁苯酞组伊文思蓝浓
度较对照组大鼠增高（P＜0.05），但较低灌注组降低（P＜0.05）。低灌注组大鼠脑白质纤维密度较对照组显著
降低（P＜0.01）；丁苯酞组大鼠脑白质纤维密度较对照组降低（P＜0.05），但较低灌注组增高（P＜0.05）。结论：
丁苯酞可减轻慢性低灌注状态导致的脑白质损害，其机制可能与改善微循环、减轻血脑屏障破坏有关。 |
| 英文摘要: |
| To estimate whether butylphthalide improves white matter damage and blood-brain barrier
disruption by chronic cerebral hypoperfusion and explore its possible mechanism. Methods: 72 Wistar rats were
randomly split into three groups with 24 in each: sham group, hypoperfusion group, and butylphthalide group. Permanent bilateral common carotid artery occlusion was used to induce hypoperfusion of the forebrain in hypoperfusion and butylphthalide group rats. Intraperitoneal injection of butylphthalide was administered to butylphthalide
group rats after surgery. Microvessel density, Kluver-Barrera’s myelin sheath staining, and quantitative measurement of pre-injected Evans Blue were performed 30 days after surgery. Results: Compared to the control and hypoperfusion group rats, the butylphthalide group rats had a higher microvessel density (P<0.05). Hypoperfusion
group rats showed a higher leakage of Evans Blue dye compared to control group rats (P<0.01). Butylphthalide
group rats showed a greater leakage of Evans Blue dye than control group rats (P<0.05) but a smaller leakage than
hypoperfusion group rats (P<0.05). Hypoperfusion group rats had a lower white matter fiber density than control
group rats (P<0.01). Butylphthalide group rats showed a lower white matter fiber density than the control group
rats (P<0.05) but a higher density than hypoperfusion group rats (P<0.05). Conclusion: Butylphthalide reduced
the white matter impairment caused by chronic cerebral hypoperfusion, and its key mechanism may involve the improvement of microcirculation and the reduction of damage to the blood brain barrier. |
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