| 张鑫瑞
，韩悦
，刘建宇
，雷蕾
，耿承奎.RGD肽修饰水凝胶携带脂肪间充质干细胞对大鼠脊髓损伤的治疗作用[J].神经损伤功能重建,2025,(6):335-339 |
| RGD肽修饰水凝胶携带脂肪间充质干细胞对大鼠脊髓损伤的治疗作用 |
| RGD Peptide Modified Hydrogel Carrying Adipose-derived Mesenchymal Stem Cells for theTreatment of Spinal Cord Injury in Rats |
| |
| DOI： |
| 中文关键词: RGD肽修饰水凝胶 脂肪间充质干细胞 脊髓损伤 大鼠 |
| 英文关键词: RGD peptide modified hydrogel adipose-derived mesenchymal stem cells spinal cord injury rat |
| 基金项目:云南省科技厅科技
计划项目（基底膜基
质胶携带脂肪基质
干细胞移植治疗大
鼠脊髓损伤，No. 20
2001AY070001-263） |
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| 摘要点击次数: 13 |
| 全文下载次数: 3 |
| 中文摘要: |
| 目的：探讨脂肪间充质干细胞（adipose-derived mesenchymal stem cells，ADSCs）联合精氨酸-甘氨酸-
天冬氨酸（Arg-Gly-Asp，RGD）肽修饰水凝胶对大鼠脊髓损伤（spinal cord injury，SCI）后再生修复的影响。
方法：将50只SD大鼠随机分为5组，假手术组（Sham组）、模型组（SCI组）、脂肪间充质干细胞组（ADSCs
组）、RGD肽修饰水凝胶组（p-Gel组）、脂肪间充质干细胞联合水凝胶组（ADSCs-pGel组），每组10只。使用
横断法制备SCI模型，各组分别在脊髓损伤部位注射生理盐水、ADSCs、RGD肽修饰水凝胶ADSCs联合
RGD 肽修饰水凝胶混合溶液，各 100 μL。在造模后第 1、3、7、14、21、28 天采用 Basso Beattie Bresnahan
（BBB）量表评对大鼠后肢运动功能进行评分；造模后第7天采用免疫荧光染色检测CD68（克隆ED-1）、胶
质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）、神经丝蛋白200（neurofilament-200，NF-200）的表达
水平。 结果：从第 7 天开始，ADSCs-pGel 组的 BBB 评分开始高于 SCI 组（P＜0.05）；从第 14 天开始，ADSCs-pGel 组的 BBB 评分高于 ADSCs 组（P＜0.05），p-Gel 组和 ADSCs 组的 BBB 评分高于 SCI 组（P＜
0.05）。各组的ED-1、GFAP荧光表达水平明显高于sham组（P＜0.001），ADSCs-pGel组的ED-1、GFAP荧光
表达水平明显低于 pGel 组和 ADSCs 组（P＜0.01）；各组的 NF-200 荧光表达水平明显低于 sham 组（P＜
0.001），ADSCs-pGel组的NF-200荧光表达水平明显高于pGel组和ADSCs组（P＜0.001）。结论：ADSCs联
合RGD肽修饰水凝胶移植到SCI部位后可促进大鼠后肢运动功能的恢复，且效果比单一应用ADSCs或
RGD肽修饰水凝胶更显著。 |
| 英文摘要: |
| To investigate the effect of adipose-derived mesenchymal stem cells (ADSCs)
combined with arginine-glycine-aspartic acid (Arg-Gly-Asp, RGD) peptide modified hydrogel on regenerative
repair after spinal cord injury (SCI) in rats. Methods: Fifty SD rats were randomly divided into five groups:
sham operation group (Sham group), model group (SCI group), adipose-derived mesenchymal stem cell group
(ADSCs group), RGD peptide-modified hydrogel group (p-Gel group), and adipose-derived mesenchymal stem
cell combined with hydrogel group (ADSCs-pGel group), with 10 rats in each group. The SCI model was
established using a transection method, and each group was injected with 100 μL of normal saline, ADSCs,
RGD peptide-modified hydrogel, or a mixture of ADSCs and RGD peptide-modified hydrogel at the site of
spinal cord injury. The Basso Beattie Bresnahan (BBB) scale was used to score the motor function of the rats'
hindlimbs on days 1, 3, 7, 14, 21, and 28 after modeling. On day 7 after modeling, immunofluorescence staining
was used to detect the expression levels of CD68 (clone ED-1), glial fibrillary acidic protein (GFAP), and
neurofilament-200 (NF-200). Results: Starting from day 7, the BBB scores of the ADSCs-pGel group were
higher than those of the SCI group (P<0.05). From day 14 onwards, the BBB scores of the ADSCs-pGel group
were higher than those of the ADSCs group (P<0.05), and the BBB scores of the p-Gel group and ADSCs group
were higher than those of the SCI group (P<0.05). The fluorescence expression levels of ED-1 and GFAP in all
groups were significantly higher than those in the Sham group (P<0.001), but the fluorescence expression levels of ED-1 and GFAP in the
ADSCs-pGel group were significantly lower than those in the p-Gel group and ADSCs group (P<0.01). The fluorescence expression level
of NF-200 in all groups was significantly lower than that in the Sham group (P<0.001), but the fluorescence expression level of NF-200 in
the ADSCs-pGel group was significantly higher than that in the p-Gel group and ADSCs group (P<0.001). Conclusion: Transplantation
of ADSCs combined with RGD peptide-modified hydrogel to the SCI site can promote the recovery of motor function in the hindlimbs of
rats, and the effect is more significant than the single application of ADSCs or RGD peptide-modified hydrogel. |
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