| Objective: To investigate the effect of adipose-derived mesenchymal stem cells ( ADSCs ) combined with RGD ( Arg-Gly-Asp ) peptide modified hydrogel on regeneration and repair after spinal cord injury ( SCI ) in rats. Methods : 50 SD rats were randomly divided into 5 groups : sham group, model group, adipose mesenchymal stem cell group, RGD peptide modified hydrogel group, adipose mesenchymal stem cell combined hydrogel group, 10 rats in each group. Spinal cord injury model was prepared by transection method. PBS was injected into the injury site after transection in the model group, ADSCs were injected into the adipose mesenchymal stem cell group, RGD peptide modified hydrogel was injected into the hydrogel group, and ADSCs combined with RGD peptide modified hydrogel was injected into the combined treatment group. BBB was used to evaluate the recovery of hind limb motor function in rats at 1, 3, 7, 14, 21，28 days after modeling. After 7 days, the expression levels of ED-1, GFAP and NF200 were detected by immunofluorescence staining. Results : The BBB score of the adipose-derived mesenchymal stem cells combined with RGD peptide hydrogel group was significantly higher than that of the model group from the 7th day ( p < 0.05 ), and was significantly higher than that of the RGD peptide modified hydrogel group and the adipose-derived mesenchymal stem cell group with time ( p < 0.05 ). The fluorescence expression levels of ED-1 and GFAP in the model group were significantly higher than those in the combined treatment group ( p < 0.05 ), and the expression of NF-200 was significantly decreased ( p < 0.05 ). Conclusion : ADSCs combined with RGD peptide modified hydrogel can promote the recovery of nerve and motor function of hind limbs in rats after transplantation to SCI site, and the effect is more significant than that of ADSCs or RGD peptide modified hydrogel alone. |