| 姚路路
，李傅尧
，倪敬年
，魏明清
，李婷
，时晶
，田金洲.经皮小脑延髓池穿刺重复抽取大鼠脑脊液的方法研究[J].神经损伤功能重建,2025,(3):125-129 |
| 经皮小脑延髓池穿刺重复抽取大鼠脑脊液的方法研究 |
| Method of Drawing Cerebrospinal Fluid from Cerebellomedullary Cistern of Rats viaPercutaneous Puncture |
| |
| DOI： |
| 中文关键词: 经皮穿刺 小脑延髓池 脑脊液 方法研究 |
| 英文关键词: percutaneous puncture cerebellomedullary cistern cerebrospinal fluid methodological study |
| 基金项目:国家自然科学基金
（补肾方对阿尔茨海
默病CD33-TREM2-
NF-κB 信号通路的
免疫调控及神经保
护作用，No. 820743
62；基于肠道菌群-
酪氨酸脱羧酶途径
探讨升清降浊方调
控左旋多巴代谢治
疗帕金森病的机制，
No. 82204921）；解
码中医协同攻关项
目（No. BZY-JMZY-
202-002）；中央高校
基本科研业务费专
项资金资助（No. 20
24-JYB-XJSJJ-008） |
|
| 摘要点击次数: 85 |
| 全文下载次数: 94 |
| 中文摘要: |
| 目的：提出一种操作简便、重复性高的方法，可用于重复采集大鼠脑脊液（cerebrospinal fluid，CSF）样
本。方法：25只SD大鼠随机分为对照组和实验组。实验组大鼠麻醉后，调整其头部相对于身体呈45°～
90°，以枕骨隆突和第一颈椎之间的中心位置为进针点，针尖向头侧，平行于大鼠头部弯曲方向缓慢刺入，
进针深度约为5 mm。缓慢抽吸CSF并记录采样量。通过观察大鼠体重变化和Bederson评分，伊文思蓝和
HE染色，检测CSF白细胞介素-6（interleukin-6，IL-6）进行综合评估。结果：实验组大鼠首次采样成功14只
（70.0%），采样量为（93.30±13.33）μL。连续二次采样成功10只（50.0%），第二次采样量为（93.00±19.86）μL。
连续三次采样成功8只（40.0%），第三次采样量为（89.75±7.72）μL。连续三次采样成功组的大鼠体重增加
（47.6±1.51）g，与对照组相比差异无统计学意义（P＞0.05）。实验组大鼠Bederson评分均为0分，提示未造
成明显神经功能缺损。未观察到明显的伊文思蓝染色和脑组织形态学改变。三次采集的CSF样本中IL-6
含量分别为（28.98±4.70）pg/mL、（29.37±6.06）pg/mL 和（30.06±5.47）pg/mL，三次对比差异无统计学意义
（P＞0.05）。第一次采样失败后再次采样成功的CSF样本IL-6含量升高（P＜0.05）。结论：经皮小脑延髓池
穿刺抽取CSF的方法所需器材简单，操作时间短，可单人操作，且对动物创伤小。 |
| 英文摘要: |
| To propose a convenient and highly reproducible method for repeat collection of rat
cerebrospinal fluid (CSF) samples. Methods: Twenty-five SD rats were randomly divided into control group
and experimental group. After anesthesia, the rats in the experimental group were positioned with their heads at
an angle of 45° to 90° relative to their bodies. A needle was inserted at the center point between the occipital
protuberance and the first cervical vertebra, with the needle tip directed toward the head and inserted slowly
parallel to the curve of the rat's head, reaching a depth of approximately 5 mm. CSF was then slowly aspirated,
and the sample volume was recorded. Comprehensive assessments were conducted by observing changes in rat
body weight and Bederson scores, as well as through Evans blue staining and hematoxylin and eosin (HE)
staining, and by measuring interleukin-6 (IL-6) levels in the CSF. Results: In the experimental group,
successful first-time sampling was achieved in 14 rats (70.0% ) with a sample volume of (93.30±13.33) μL.
Successful consecutive second-time sampling was achieved in 10 rats (50.0%) with a sample volume of (93.00±
19.86) μL. Successful consecutive third-time sampling was achieved in 8 rats (40.0%) with a sample volume of
(89.75±7.72) μL. Rats in the group with successful consecutive three-time sampling showed an increase in body
weight of (47.6±1.51) g, which was not statistically significant compared to the control group (P>0.05). The
Bederson scores of the rats in the experimental group were all 0, indicating no significant neurological deficits.
No obvious Evans blue staining or morphological changes in brain tissue were observed. The IL-6 levels in the
CSF samples collected during the three samplings were (28.98±4.70) pg/mL, (29.37±6.06) pg/mL, and (30.06±
5.47) pg/mL, respectively, with no statistically significant differences among the three collections (P>0.05).
However, an increase in IL-6 levels was observed in CSF samples successfully collected after an initial failed
attempt (P<0.05). Conclusion: The method of percutaneous cerebellomedullary cistern puncture for CSF
collection requires simple equipment, has a short operating time, can be performed by a single individual, and
causes minimal trauma to the animals. |
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