| 来小音，
，毕抓劲
，杨雪莲
，张瀚文
，李龙宣
，卜碧涛.支链氨基酸在重症肌无力患者血中的变化及其免疫调节作用研究[J].神经损伤功能重建,2022,17(1):9-12 |
| 支链氨基酸在重症肌无力患者血中的变化及其免疫调节作用研究 |
| Changes of Branched-Chain Amino Acids in Patients with Myasthenia Gravis and TheirImmunomodulatory Effects |
| |
| DOI： |
| 中文关键词: 重症肌无力 支链氨基酸 细胞因子 实验性自身免疫性重症肌无力 |
| 英文关键词: myasthenia gravis branched-chain amino acids cytokines experimental autoimmune
myasthenia gravis |
| 基金项目:上海市浦东新区科
技发展基金（No. P
KJ2018-Y07）；
上海市医学重点专
科项目（No. ZK201
9A08）；
国家自然科学基金
（No. 8187051428）；
2018 年 上 海 市 浦
东新区公利医院青
年 英 才 培 养 计 划
（No. kyk_2019_00
31） |
|
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| 中文摘要: |
| 目的：分析支链氨基酸（BCAAs）在重症肌无力(MG)患者外周血中的变化，探讨其在实验性自身免疫
性重症肌无力（EAMG）模型中的免疫调节作用。方法：收集54例MG患者纳入MG组，另设35例健康体检
者为对照组，采用液相色谱-质谱分析检测血中BCAAs水平、淋巴细胞亚群和细胞因子，比较2组的差异。
使用BCAAs喂养EAMG小鼠，评估其肌力变化，并采用ELISA方法检测细胞因子水平。结果：与对照组相
比，MG组患者血缬氨酸、异亮氨酸水平降低，而TNF-α、IL-6、IL-8水平升高（P＜0.05）。与正常饮食组相
比，BCAAs饮食组小鼠症状较轻且TNF-α、IL-6、IL-8水平降低（P＜0.05）。结论：BCAAs有益于改善MG的
免疫反应，通过调节BCAAs的代谢或其相关的转运蛋白及信号通路有可能成为调节MG免疫的新切入点。 |
| 英文摘要: |
| To analyze the change of branched chain amino acids (BCAAs) level in peripheral
serum of patients with myasthenia gravis (MG), and to explore its immunomodulatory role in the experimental
autoimmune myasthenia gravis (EAMG) animal model. Methods: Total 54 MG patients (MG group) and 35
healthy controls (control group) were enrolled respectively. Liquid chromatography - mass spectrometry testing
was used to detect the levels of BCAAs, lymphocyte subsets, and cytokines in both groups, and the results were
compared. EAMG mice were fed by BCAAs, then changes to muscle strength were evaluated and cytokine
levels were detected by ELISA. Results: Compared with those of the control group, the levels of Val and Ile in
the MG group were lower, and the levels of TNF-α, IL-6, IL-8 were higher (P<0.05). Compared with normal
diet EAMG mice, the BCAA-fed mice showed significantly reduced clinical scores and levels of TNF-α, IL-6,
and IL-8 (P<0.05). Conclusion: BCAAs are helpful in improving immune response in MG. Through
adjustments to BCAAs metabolism or their transport proteins, a new route for regulating immunity in MG may
be established. |
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