| 聂颖
，范瑜洁
，王枭冶
，范婷婷
，万彩云
，李亮.补阳还五汤联合骨髓间充质干细胞移植对脊髓损伤气虚血瘀证的疗效[J].神经损伤功能重建,2021,16(12):714-718 |
| 补阳还五汤联合骨髓间充质干细胞移植对脊髓损伤气虚血瘀证的疗效 |
| Therapeutic Effect of Buyang Huanwu Decoction Combined with BMSCs Transplantation onSpinal Cord Injury with Chinese Medicine Pattern of Qi Deficiency and Blood Stasis |
| |
| DOI： |
| 中文关键词: 补阳还五汤 骨髓间充质干细胞 脊髓损伤 气虚血瘀证 |
| 英文关键词: Buyang Huanwu decoction bone marrow mesenchymal stem cells spinal cord injury qi deficiency and blood stasis |
| 基金项目:湖南省自然科学基
金资助项目（No. 2
018JJ2289，2019JJ
50310, 2019JJ8007
1）；
湖南省中医药科研
计划项目（No. 202
083）；
湖南中医药大学中
医学一流学科开放
基金项目（No. 201
8ZYX01） |
|
| 摘要点击次数: 1873 |
| 全文下载次数: 2187 |
| 中文摘要: |
| 目的：探讨补阳还五汤（BYHWD）联合骨髓间充质干细胞（BMSCs）移植对脊髓损伤（SCI）气虚血瘀
证的疗效，并探讨其分子机制。方法：将120只雄性SD大鼠随机分为5组：对照组、气虚血瘀组、BYHWD
组、BMSCs组、BYHWD+BMSCs组，每组24只。制作SCI气虚血瘀证复合动物模型，并分别予以BYHWD、
BMSCs或BYHWD+BMSCs治疗28 d。治疗前、治疗后1 d、14 d、28 d通过自发性直立探究行为观察前肢运
动功能恢复情况；治疗后28 d，ELISA法检测脊髓损伤局部cAMP浓度，HE染色观察损伤局部轴突形态，免
疫组织化学法检测脊髓损伤局部RhoA、神经生长因子（NGF）、脑源性神经营养因子（BDNF）的表达。结 果：治疗后，BYHWD 组、BMSCs 组和 BYHWD+BMSCs 组大鼠的前肢使用率均高于气虚血瘀组，脊髓
cAMP 含量均高于气虚血瘀组（均 P＜0.01），且 BYHWD+BMSCs 组高于 BYHWD 组和 BMSCs 组（均 P＜ 0.05 或 P＜0.01）。HE 染色显示气虚血瘀组、BMSCs 组脊髓右后外侧索轴突水肿或缺失，髓鞘排列散乱；
BYHWD组、BYHWD+BMSCs组脊髓损伤程度减轻。与气虚血瘀组相比，BYHWD组、BYHWD+BMSCs组
NGF、BDNF阳性表达升高，BMSCs组BDNF阳性表达升高，RhoA表达下降（P＜0.05或P＜0.01）；且BYHWD+BMSCs组BDNF表达水平高于BYHWD组和BMSCs组（P＜0.05），RhoA表达水平低于BYHWD组和
BMSCs组（P＜0.05）。结论：BYHWD联合BMSCs移植对保护SCI气虚血瘀证脊髓局部轴突、改善前肢运
动功能可产生协同增效作用，其作用机制可能与上调脊髓局部微环境cAMP浓度，抑制RhoA表达，上调
NGF、BDNF有关。 |
| 英文摘要: |
| To examine the therapeutic effect of Buyang Huanwu Decoction (BYHWD) combined
with bone marrow mesenchymal stem cells (BMSCs) transplantation on spinal cord injury (SCI) with Chinese
medicine pattern of qi deficiency and blood stasis, and to explore its mechanism. Methods: A total of 120 male
SD rats were randomly divided into the following five groups with 24 rats in each: the control, qi deficiency and
blood stasis, BYHWD, BMSC, and BYHWD+BMSCs groups. SCI with qi deficiency and blood stasis rat models were established, and BYHWD, BMSC, or BYHWD+ BMSC treatment was administered for a duration of
28 days. Recovery of forelimb motor function was assessed by observing spontaneous vertical exploration before
treatment and at 1, 14, and 28 days of treatment. After 28 days of treatment, we performed the ELISA test to measure cAMP concentration in the spinal cord, HE staining to observe the local axon morphology in areas of damage, and immunohistochemistry to detect the local expression of RhoA, nerve growth factor (NGF), and brain-derived neurotrophic factor (BDNF). Results: After treatment, rats in the BYHWD, BMSC, and BYHWD+BMSC
groups showed greater usage rate of forelimbs and higher spinal cord concentrations of cAMP compared to the qi
deficiency and blood stasis group rats (all P<0.01); furthermore, the BYHWD+BMSC group displayed greater results than the BYHWD and BMSC groups (all P<0.05 or P<0.01). HE staining revealed that, in the qi deficiency
and blood stasis group and BMSC group, rubrospinal tract (RST) axons suffered edema or disappeared, and myelin sheaths became disorderly arranged. In the BYHWD and BYHWD+BMSC groups, there was a reduction in
the degree of spinal cord injury. Immunohistochemistry showed that, compared with that in the qi deficiency and
blood stasis group, expression of NGF and BDNF in the BYHWD and BYHWD+BMSC groups increased; expression of BDNF in the BMSC group increased and that of RhoA decreased (P<0.05 or P<0.01). Additionally,
expression of BDNF was higher and that of RhoA was lower in the BYHWD+ BMSC group compared to the
BYHWD and BMSC groups (P<0.05). Conclusion: BYHWD combined with BMSC transplantation can produce a synergistic effect on
the protection of local axons in SCI with qi deficiency and blood stasis and on the improvement of forelimb motor function. The mechanism may be related to up-regulating cAMP concentration, inhibiting RhoA expression, and up-regulating NGF and BDNF expression in
the spinal cord microenvironment. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|