文章摘要
徐林 ，甄丽晓 ，齐宏顺, ，李培培 ，吕涌涛, ，冯肖亚 ，葛汝村.不同剂量尿激酶对大鼠脑出血后血脑屏障作用的研究[J].神经损伤功能重建,2021,16(6):323-325
不同剂量尿激酶对大鼠脑出血后血脑屏障作用的研究
Protective Effects of Different Doses of Urokinase on Blood-Brain Barrier in Rat Model of In⁃tracerebral Hemorrhage
  
DOI：
中文关键词: 脑出血  尿激酶  紧密连接蛋白1  基质金属蛋白酶9
英文关键词: intracerebral hemorrhage  urokinase  tight junction protein 1  matrix metalloproteinase 9
基金项目:山东省医药卫生 科技发展计划项 目（No. 2016WS0 396）
作者单位
徐林a ，甄丽晓a ，齐宏顺a,b ，李培培a ，吕涌涛a,b ，冯肖亚b ，葛汝村a 山东省立第三医 院 a. 转化医学研 究中心，b. 神经 内科 
摘要点击次数: 2669
全文下载次数: 3389
中文摘要:
      目的：比较不同剂量尿激酶对大鼠脑出血（ICH）后血脑屏障（BBB）的影响及机制。方法：成年雄性Wistar大鼠40只，经尾状核注射自体血建立ICH模型，造模成功后，40只大鼠随机分为ICH组和低（3万U/mL）、 中（5万U/mL）、高剂量（10万U/mL）尿激酶组（L、M、H组），每组10只。造模成功3 h后，将不同剂量的尿激 酶分别注入血肿处，模型对照组给予等量生理盐水。造模后3 d，采用干湿重法检测血肿周围脑组织含水量， 伊文斯蓝染色检测BBB渗透性，Western blot检测血肿周围脑组织紧密连接蛋白（ZO-1）和基质金属蛋白酶9 （MMP-9）的表达变化。结果：与ICH组比较，L、M和H组大鼠血肿周围脑组织含水量降低（均P＜0.05），大鼠 脑组织的EB染料血管外渗出减少（均P＜0.05），脑组织ZO-1表达明显升高（均P＜0.05），MMP-9的表达明显 降低（均P＜0.05）；且L组（300 U尿激酶）的血肿周围脑组织含水量最低，EB染料血管外渗出最少，脑组织 ZO-1表达最高，MMP-9的表达最低，与M和H组差异均有统计学意义（均P＜0.05）。结论：300 U尿激酶血肿 腔内注射治疗能保护ICH后BBB的完整性，其机制可能与ZO-1表达增加及MMP-9表达降低有关。
英文摘要:
      To compare the effects and mechanism of different doses of urokinase on the blood-brain barrier (BBB) in rats of intracerebral hemorrhage (ICH). Methods: We selected 40 adult male Wistar rats and established the ICH model by autologous blood injection into the caudatum. After successful modeling, they were randomly divided into 4 groups, each with 10 rats: the control group and the low dose (30000 U/mL), medium dose (50000 U/mL), and high dose (100000 U/mL) urokinase groups, respectively named the ICH group and L, M, and H dose groups. Three hours after establishment of the model, different doses of urokinase were injected into the hematoma respectively, and the rats in the ICH group received the same amount of normal saline. Three days after modeling, the brain water content was measured by the dry/wet weight method, the BBB permeability was evaluated by Evans blue fluorescence, and the expression levels of zonula occludens-1 (ZO-1) and matrix metalloproteinase-9 (MMP-9) in brain tissues around the hematoma were detected by Western blot. Results: Compared with the ICH group, the L, M, and H dose groups showed a decreased water content in brain tissues surrounding the hematoma (all P<0.05), reduced EB dye permeation out of blood vessels in brain tissues (all P<0.05), and up-regulated ZO-1 (all P<0.05) and down-regulated MMP-9 (all P<0.05) in brain tissues. Furthermore, the L dose group exhibited the lowest water content in tissues surrounding the hematoma, the least EB dye seepage from blood vessels, the highest ZO-1 expression, and the lowest MMP-9 expression, with results being significantly different from those of the M and H dose groups (all P<0.05). Conclusion: The injection of 300 U urokinase into hematoma serves to protect the integrity of the BBB after ICH, and the mechanism may involve increasing ZO-1 and decreasing MMP-9 expression.
查看全文   查看/发表评论  下载PDF阅读器
关闭