| 刘莹莹，刘伟.不同频率督脉电针促进不完全脊髓损伤大鼠运动功能重建的比较研究[J].神经损伤功能重建,2021,16(4):191-194 |
| 不同频率督脉电针促进不完全脊髓损伤大鼠运动功能重建的比较研究 |
| A Comparative Study of Du Meridian Electroacupuncture with Different Frequencies in Pro⁃moting Reconstruction of Motor Function in Rats with Incomplete Spinal Cord Injury |
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| DOI： |
| 中文关键词: 督脉电针 不完全脊髓损伤 痛超敏行为学 凝血功能 痛阈 运动功能 |
| 英文关键词: Du meridian electroacupuncture incomplete spinal cord injury hyperalgesia behavior blood coagulation function pain threshold motor function |
| 基金项目:湖北省十堰市太和
医院院级课题（No.
2018JJXM120） |
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| 中文摘要: |
| 目的：比较低、中、高频督脉电针对不完全脊髓损伤（iSCI）模型大鼠运动功能的影响。方法：采用随机
数字表法将50只Wistar大鼠随机均分为假手术组，模型组和低、中、高频电针组（G1、G2、G3组），除假手术
组外（仅行椎板全切除）均采用美国NYU脊髓冲击损伤仪制作大鼠T11节段iSCI模型。G1、G2、G3组分别用低
频（2 Hz，2 mA）、中（50 Hz，2 mA）、高频（100 Hz，2 mA）电针“大椎、命门”30 min，1次/d，共治疗2周。观察各
组大鼠痛超敏行为学并采用von Frey hair法进行痛阈测定；记录股二头肌（BF）和股直肌（RF）的肌电积分值
（IEMG）BF-TEMG和RF-TEMG；采用玻片法和断尾法观凝血时间（CT) 和出血时间（BT）；采用凝血因子生成
实验检测凝血酶、内/外源性凝血因子Xa活性；采用Basso Beattie Bresnahan（BBB）评分和斜板实验评价运动
功能恢复情况。结果：与模型组相比，G1、G2和G3组大鼠BF-TEMG和RF-TEM升高，内/外源性凝血因子
Xa和凝血酶生成明显降低，CT和BT延长，对损伤躯干下端机械性轻触皮肤、轻压皮肤、机械轻压右前爪的
机械痛痛阈阈值等3项指标提高，搔抓、舔咬损伤平面以下部位并对尾部及后肢出现自噬及自发嘶叫等异常
行为学改变明显减少，BBB 评分和斜板倾斜度升高（P＜0.05）；但 G1 组的各项改变幅度均大于 G2、G3 组 （P＜0.05）。结论：低、中、高频电针均可促进iSCI大鼠运动功能重建，但低频督脉电针的作用优于中、高频。 |
| 英文摘要: |
| Compare the effects of low, medium, and high frequency Du meridian acupuncture on
the motor function of rats with incomplete spinal cord injury (iSCI). Methods: Using the random number table
method, 50 Wistar rats were randomly divided into the sham operation group; model group; and low, medium,
and high frequency electroacupuncture groups (G1, G2, and G3 groups). The iSCI model of the rat T11 segment
was made with the NYU spinal cord impact injury device for all groups aside from the sham operation group
(which only underwent a total lamina resection). The G1, G2, and G3 groups were treated with low (2 Hz, 2 mA),
medium (50 Hz, 2 mA), and high frequency (100 Hz, 2 mA) electroacupuncture at the Dazhui and Mingmen
points for 30 minutes once a day for a total of 2 weeks. We observed the behaviors of pain hypersensitivity in rats
in each group and used the von Frey hair method to measure pain threshold. We recorded the biceps femoris (BF)
and rectus femoris (RF) myoelectric integral value (IEMG), BF-TEMG and RF-TEMG, respectively. The slide
method and tail-docking method were used to observe clotting time (CT) and bleeding time (BT). The coagulation factor test was conducted to detect thrombin and endogenous/exogenous coagulation factor Xa activity. The
Basso Beattie Bresnahan (BBB) score and inclined board test were used to evaluate the recovery of motor function. Results: Compared with the model group, the G1, G2, and G3 groups showed an increase in BF-TEMG
and RF-TEM; decrease in production of endogenous/exogenous coagulation factor Xa and thrombin; prolonged
CT and BT; increased pain threshold in 3 indexes including light touch and light pressure on the skin in the lower
portion of the injured trunk and light pressure on the right forepaw; reduction in scratching, licking and biting the
area below the plane of injury, appearance of autophagy on the tail and hind limbs, and spontaneous hissing; and
increased BBB score and inclined plate slope (P<0.05). Furthermore, the G1 group showed a greater difference in
each of the above measurements compared to the G2 and G3 groups (P<0.05). Conclusion: Low, medium, and
high frequency electroacupuncture can promote the reconstruction of motor function in rats with iSCI, but the effect of low frequency Du meridian electroacupuncture is superior to that of medium and high frequencies. |
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