文章摘要
陈梓杰 ，陈柏行 ，许隆 ，张严 ，冯俊铭 ，李世杰 ，高怡加 ，曾展鹏.神经源性异位骨化相关差异基因的筛选及生物信息学分析[J].神经损伤功能重建,2021,16(2):71-75
神经源性异位骨化相关差异基因的筛选及生物信息学分析
Identification and Bioinformatics Analysis of Differential Genes Related to Neurogenic Hetero⁃topic Ossification
  
DOI：
中文关键词: 神经源性异位骨化  差异表达基因  生物信息学  蛋白质相互作用
英文关键词: neurogenic heterotopic ossification  differentially expressed gene  bioinformatics  protein-protein interaction
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作者单位
陈梓杰1 ，陈柏行1 ，许隆1 ，张严1 ，冯俊铭1 ，李世杰1 ，高怡加2 ，曾展鹏2 1. 广州中医药大 学第一临床医学 院 2. 广州中医药大 学附属第一医院 一骨科 
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中文摘要:
      目的：基于GEO数据库筛选神经源性异位骨化的差异基因并进行生物信息学分析，寻找疾病相关基 因和通道。方法：从GEO数据库中下载关于神经源性异位骨化的基因表达谱芯片，通过R软件的Limma包 以调整后的P＜0.05和|Log2(fold-change)|＞2作为阈值筛选异位骨化组和健康对照组的差异基因，使用Matascape在线工具对差异基因进行GO富集及KEGG通路富集分析，并构建差异基因蛋白质-蛋白质相互作用 关系（PPI）网络，利用Cytohubba和MCODE算法寻找关键基因。结果：共筛选出276个差异基因，其中上调 基因150个，下调基因126个，差异表达基因的生物学过程（BP）主要在化学突触传递、跨突触信号的调控、骨 化等过程富集；分子功能（MF）主要在细胞外基质结构成分、肝素结合、糖胺聚糖结合富集；细胞成分（CC）主 要在胶原三聚体、含胶原蛋白的细胞外基质、细胞外基质富集。KEGG信号通路分析主要富集在补体和凝血 级联、IL-17、Wnt、蛋白的消化吸收等通路。PPI网络共鉴别出EGF、GPR18、ADRA2C、CXCL1、C3、CXCL6、 ADRB2、PENK、CXCL2、CDH1 共10个hub基因和CXCL2、PTH2、EPHB1、HTR2B 共4个种子基因。结论： 通过对基因芯片的生物信息学分析，发现了可能与神经源性异位骨化相关的基因及分子调控机制。
英文摘要:
      To identify the differential expressed genes of neurogenic heterotopic ossification (NHO) based on GEO database and analyze them by bioinformatics to find the disease-related genes and channels. Meth⁃ ods: Download the gene expression microarray about NHO from GEO database. The differential genes of NHO group and healthy control group were screened by Limma package of R Software with the thresholds of adj. P-value < 0.05 and | Log 2 (fold-change) | > 2. The differential genes were enriched by GO and KEGG pathway by Matascape online tool, and the network of protein-protein interaction (PPI) of differential genes was constructed. Cytohubba and Mcode algorithms were used to find the key genes. Results: A total of 276 differential genes were screened, including 150 up-regulated genes and 126 down-regulated genes. The biological process (BP) of differentially expression genes was mainly enriched in modulation of chemical synaptic transmission, regulation of trans-synaptic signaling and ossification. Molecular function (MF) was mainly enriched in extracellular matrix structural constituent, heparin binding and glycosaminoglycan binding. The cellular component (CC) was mainly enriched in collagen trimer, collagen-containing extracellular matrix and extracellular matrix. KEEG signal pathway analysis was mainly enriched in complement and coagulation cascade, IL-17 signal pathway, Wnt signal pathway, protein digestion and absorption and so on. Ten hub genes including EGF, GPR18, ADRA2C, CXCL1, C3, CXCL6, ADRB2, PENK, CXCL2, CDH1 and four seed genes including CXCL2, PTH2, EPHB1, HTR2B were identified in PPI network. Conclusion: Through the bioinformatics analysis of the gene microarray, we found the genes and molecular regulation mechanisms that may be related to NHO.
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