| 魏文洁，
，苏醒
，谢敏杰
，王伟
，骆翔
，喻志源.光基因技术选择性调控星形胶质细胞内钙离子水平的研究[J].神经损伤功能重建,2020,15(4):187-189 |
| 光基因技术选择性调控星形胶质细胞内钙离子水平的研究 |
| Effect of Selective Optogenetic Regulation on Astrocyte Intracellular Calcium |
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| DOI： |
| 中文关键词: 光基因技术 光敏感通道2 星形胶质细胞 钙离子 |
| 英文关键词: optogenetics Channelrhodopsin2 astrocyte Ca2+ |
| 基金项目:国家自然科学基
金 面 上 项 目（No.
81771341）；
国家自然科学基
金 青 年 项 目（No.
81600990）；
湖北省自然科学
基金（No. 2017CF
B705） |
|
| 摘要点击次数: 2713 |
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| 中文摘要: |
| 目的：研究光敏感通道蛋白（ChR2）选择性调控星形胶质细胞，对其细胞内钙离子水平的影响。方法：
离体培养SD大鼠星形胶质细胞细胞，采用慢病毒载体Lenti-GFAP-ChR2-YFP感染星形胶质细胞后，钙离子
荧光探针Rhod-2 AM作为钙离子活动的指示，以470 nm波长的蓝光刺激星形胶质细胞，实时在共聚焦荧光
下检测星形胶质细胞钙离子活动。结果：成功将光敏感通道蛋白（ChR2）表达在星形胶质细胞；激活光敏感
通道ChR2后，引起星形胶质细胞内钙信号活动，钙离子水平增加。结论：以光敏感通道ChR2为核心的光基
因技术可运用于调控星形胶质细胞钙离子活动。 |
| 英文摘要: |
| To study the effect of selective optogenetic regulation by Channelrhodopsin2 (ChR2) on
intracellular calcium levels in astrocytes. Methods: Astrocytes were cultured from SD neonate rats, and a lentiviral vector, Lenti-GFAP-ChR2-YFP, was used to transduce cultured astrocytes. Astrocytes were stimulated using blue light with a wavelength of 470 nm, and intracellular calcium signals, as indicated by the fluorescent Ca2+
indicator Rhod-2 AM, were recorded in real time by confocal fluorescence microscopy. Results: ChR2 was successfully expressed in astrocytes. Photostimulation of ChR2 in astrocytes led to the activation of intracellular calcium signaling and an increase in calcium levels. Conclusion: The use of ChR2 as an essential element of optogenetics could be a powerful tool in the regulation of calcium in astrocytes. |
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