文章摘要
陈朋 ，刘睿 ，骆翔 ，喻志源 ，王伟 ，陈雪.小胶质细胞培养上清液对鼻咽癌细胞迁移和侵袭能力的影响[J].神经损伤功能重建,2020,15(3):125-128
小胶质细胞培养上清液对鼻咽癌细胞迁移和侵袭能力的影响
Effect of Microglia Cell Supernatant on Migration and Invasion of Nasopharyngeal CarcinomaCells
  
DOI：
中文关键词: 人小胶质细胞  鼻咽癌细胞  迁移  侵袭
英文关键词: microglia  nasopharyngeal carcinoma  migration  invasion
基金项目:国家自然科学基 金（No.81771341）； 湖北省自然科学 基金（No.2017CFB 705）
作者单位
陈朋1 ，刘睿1 ，骆翔1 ，喻志源1 ，王伟1 ，陈雪2 1. 华中科技大学 同济医学院附属 同济医院神经内 科 2. 华中科技大学 同济医学院附属 武汉儿童医院神 经内科 
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中文摘要:
      目的：研究未活化和活化态人小胶质细胞系CHME-5培养上清液对鼻咽癌细胞CNE-2迁移、侵袭能 力的影响。方法：采用1 μg/mL LPS活化人小胶质细胞系CHME-5，收集未活化和LPS活化的CHME-5培养 上清液。分为对照组（无血清培养基）、45%上清组、90%上清组、LPS组（LPS干预的小胶质细胞上清）、45% LPS上清组、90%LPS上清组。使用划痕实验和Transwell小室建立鼻咽癌细胞CNE-2的迁移模型，观察不 同浓度的未活化/活化小胶质细胞培养上清液对鼻咽癌细胞迁移能力的影响；使用Transwell小室建立鼻咽 癌细胞CNE-2的侵袭模型，用结晶紫染色观察不同浓度的未活化/活化小胶质细胞培养上清液对鼻咽癌细 胞侵袭能力的影响。结果：低浓度（45%）和高浓度（90%）未活化/活化的小胶质细胞培养上清液均促进鼻咽 癌细胞迁移和侵袭。结论：无论小胶质细胞是否活化，其培养上清液均促进鼻咽癌细胞的迁移和侵袭。
英文摘要:
      To study the effect of unactivated/activated human microglia cell line CHME-5 on the migration and invasion of nasopharyngeal carcinoma cell line CNE-2. Methods: Human microglia cell line CHME-5 was cultured, and 1 μg/mL LPS was used to activate cells. Unactivated/activated cell supernatant was collected. For the experiment, we established 6 groups: control group, 45% supernatant group, 90% supernatant group, LPS group (LPS activated microglia supernatant), 45% LPS supernatant group, and 90% LPS supernatant group. The wound healing assay and Transwell inserts were used to establish the nasopharyngeal carcinoma cell line CNE-2 migration model, and the effect of unactivated/activated microglia supernatant on the migration of the cell was observed. Transwell inserts were used to establish the nasopharyngeal carcinoma cell line CNE-2 invasion model, and crystal violet staining was used to observe the effect of microglia supernatant on the migration of the cell. Results: Low (45%) and high (90%) concentrations of unactivated/activated microglia supernatant promoted the migration and invasion of nasopharyngeal cancer cells. Conclusion: Both unactivated and activated microglia supernatant promote the migration and invasion of nasopharyngeal carcinoma cells.
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