| 熊颖
，张强
，潘邓记.星形胶质细胞特异性敲除Adam10基因对周细胞和
血脑屏障的影响[J].神经损伤功能重建,2019,14(3):109-112 |
| 星形胶质细胞特异性敲除Adam10基因对周细胞和
血脑屏障的影响 |
| Effects of Knockout Astrocytic Adam10 Gene on Pericytes and Blood Brain Barrier |
| |
| DOI： |
| 中文关键词: 星形胶质细胞 周细胞 Adam10 血脑屏障 激光散斑衬比成像 |
| 英文关键词: astrocyte pericyte Adam10 blood brain barrier laser speckle imaging |
| 基金项目:国家自然科学基
金（No. 81471230；
No. 81601480；
No. 81571206） |
|
| 摘要点击次数: 2800 |
| 全文下载次数: 2870 |
| 中文摘要: |
| 目的：探讨Adam10基因对于星形胶质细胞与周细胞之间信号交流及对血脑屏障（BBB）完整性的影
响。方法：利用Cre-loxp技术得到星形胶质细胞特异性敲除Adam10基因小鼠（Gfap
cre
-Adam10
lox/lox
）纳入敲除
组，将同胎基因型为Adam10
lox/lox
的小鼠纳入对照组。免疫荧光双标技术检测周细胞心肌素（myocardin）蛋白
和平滑肌肌动蛋白（SMA）的表达；电镜分析周细胞的变性和凋亡、基底膜和BBB的破坏；激光散斑衬比成
像技术观察脑组织局部血流灌注的改变。结果：特异性敲除星形胶质细胞Adam10基因后，可影响相邻的星
形胶质细胞和周细胞之间的信号交流，导致周细胞表达myocardin蛋白和SMA蛋白减少；敲除组呈现出更
为明显的周细胞变性、凋亡和BBB破坏；进而影响血管密度和血流速度。结论：Adam10基因可能影响星形
胶质细胞与周细胞之间的信号交流，并可能因此而影响BBB完整性和脑组织的血流灌注。 |
| 英文摘要: |
| To investigate the effect of Adam10 gene on the cross-talk between astrocyte and pericyte, and on the integrity of blood-brain barrier (BBB). Methods: Adam10 gene knockout mice (Gfap
cre
-Adam10
lox/lox
) were obtained by Cre-loxp technique. Adam10
lox/lox mice were used as control. The expression of myocardin and smooth muscular actin (SMA) protein in pericytes was detected by double immunofluorescence labeling technique. The degeneration and apoptosis of pericytes, destruction of basement membrane and BBB were analyzed by electron microscopy. Laser speckle imaging was used to observe the changes of regional cerebral blood
perfusion. Results: Specific knockout of Adam10 gene in astrocytes affectted the signal exchange between astrocyte and pericyte; decreased the myocardin and SMA protein expression in pericytes; induced pericyte degeneration and apoptosis; damaged BBB; and affected vascular density and blood flow velocity in brain cortex. Conclu⁃
sion: Adam10 gene may affect the signal exchange between astrocytes and pericytes, thus affecting the integrity
of BBB and cerebral blood perfusion. |
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