| 史文涛
，戴瑶
，毕士奇
，陆浩
，吕德民
，张志坚.氯倍他索促进鼻粘膜间充质干细胞成神经样细胞分化[J].神经损伤功能重建,2019,14(1):1-5 |
| 氯倍他索促进鼻粘膜间充质干细胞成神经样细胞分化 |
| Effect of Clobetasol on Differentiation of Nasal Ectomesenchymal Stem Cells intoNeuron-Like Cells |
| |
| DOI： |
| 中文关键词: 鼻粘膜外胚层间充质干细胞 氯倍他索 神经样细胞 音猬因子 |
| 英文关键词: nasal ectomesenchymal stem cells Clobetasol neuron-like cells sonic hedgehog |
| 基金项目:国家自然科学基金
地区科学基金（No.
81571830）；
镇江市科技局发展
项目（No. SH20080
44） |
|
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| 中文摘要: |
| 目的：研究氯倍他索（Clo）对鼻粘膜外胚层间充质干细胞（EMSCs）向神经样细胞分化的影响。方法：
贴壁筛选法分离培养EMSCs，免疫荧光法鉴定体外扩增的EMSCs；MTT法检测Clo对EMSCs增殖能力的
影响；成神经样细胞诱导实验分为Clo组、音猬因子（SHH）组和空白对照组，采用全反式维甲酸（ATRA）诱
导各组细胞向神经样细胞分化；倒置显微镜观察细胞形态的变化；免疫荧光和免疫印迹方法检测神经元细
胞相关蛋白。结果：体外分离培养的 EMSCs 高表达间充质干细胞和神经嵴源干细胞标志物 Vimentin 和
Nestin。各组EMSCs成神经样细胞诱导7 d后，Clo组和SHH组EMSCs呈现双极和多极的典型神经样细胞
形态并且高表达神经元细胞相关蛋白。Clo 组 EMSCs 表达 SHH 和 SMO 的水平明显高于空白对照组，与
SHH组比较差异无统计学意义（P>0.05）；Clo可促进EMSCs分泌内源性脑神经营养因子（BDNF）和神经生
长因子（NGF）。结论：Clo可通过激活SHH信号通路和上调内源性神经营养因子水平促进EMSCs向神经样
细胞分化。 |
| 英文摘要: |
| To study the effect of Clobetasol propionate (Clo) on the differentiation of nasal
mesenchymal stem cells (EMSCs) into neuron-like cells. Methods: EMSCs were isolated and cultured by the
adherent screening method, and the EMSCs amplified in vitro were identified by immunofluorescence. The
effect of Clo on the proliferation of EMSCs was examined by MTT assay. Induced neuroblast-like cells were
divided into the Clo group, Sonic hedgehog (SHH) group, and control group. All-trans retinoic acid (ATRA) was
used to induce differentiation into neuron-like cells. Changes in cell morphology were observed under inverted
microscope, and detection of neuronal cell-associated proteins was accomplished by immunohistochemistry and
immunoblot methods. Results: EMSCs isolated and cultured in vitro displayed high expression levels of
mesenchymal and neural stem cell markers vimentin and nestin. After induction of EMSCs into neuroblast-like
cells for 7 days, the EMSCs in the Clo and SHH groups showed bipolar and multipolar typical neuron-like cell
morphology and high expression of neuronal cell-associated proteins. At the same time, the expression levels of
SHH and SMO in the EMSCs of the Clo group were significantly higher than those of the control group, and
there was no significant difference compared with that of the SHH group (P>0.05). Data showed that Clo could
promote the secretion of endogenous brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF)
by EMSCs. Conclusion: Clo can promote the differentiation of EMSCs into neuron-like cells by activating the
SHH signaling pathway and up-regulating the levels of endogenous neurotrophic factors. |
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