| 龙根
，谢敏杰
，王伟
，徐沙贝
，刘晨辰.Cdk5及p35在星形胶质细胞正常及缺血再灌注后的
表达研究[J].神经损伤功能重建,2018,13(4):163-165 |
| Cdk5及p35在星形胶质细胞正常及缺血再灌注后的
表达研究 |
| The Expression of Cdk5 and p35 in Primary Cultured Astrocytes and the Changes inExpression after Ischemia/Reperfusion |
| |
| DOI： |
| 中文关键词: 星形胶质细胞 Cdk5 p35 缺血再灌注 |
| 英文关键词: astrocyte Cdk5 p35 oxygen-glucose deprivation/reperfusion |
| 基金项目:华中科技大学同济医
学院附属同济医院
科研基金资助项目
（No. 2201101919）；
华中科技大学同济
医学院研究型临床
医师资助计划
（No.5001540023） |
|
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| 中文摘要: |
| 目的：研究细胞周期蛋白依赖性激酶（Cdk）5和p35在星形胶质细胞中的表达及缺血再灌注后的其表
达变化趋势。方法：离体培养SD大鼠星形胶质细胞，通过免疫荧光染色观察Cdk5和p35在星形胶质细胞中
的表达分布。对培养的星形胶质细胞进行糖氧剥夺/恢复（OGD/R）处理，Western blot检测Cdk5和p35在星
形胶质细胞OGD/R后不同时间点的表达变化。结果：Cdk5和p35均在星形胶质细胞中存在表达，以胞浆为
主，胞核表达较少；两者表达分布一致。OGD/R后Cdk5和p35的表达呈早期显著上升，后期下降的趋势。
结论：Cdk5和p35在星形胶质细胞中均存在表达，以胞浆为主，且两者表达分布基本一致。OGD/R后星形
胶质细胞中的Cdk5和p35的表达呈动态变化。 |
| 英文摘要: |
| To investigate the expression of Cdk5 and p35 in primary cultured astrocytes and the
changes in expression induced by ischemia/reperfusion. Methods: Astrocytes were cultured from SD neonate
rats. The expression and distribution of Cdk5 and p35 in cultured astrocytes were analyzed by
immunofluorescence staining. Primary cultured astrocytes were subjected to oxygen-glucose deprivation/
reperfusion (OGD/R), and the changes in Cdk5 and p35 expression after OGD/R were observed by Western blot.
Results: Cdk5 and p35 were expressed in astrocytes, mainly in the cytoplasm and less in the nucleus, and both
displayed similar cellular distribution. The expression of Cdk5 and p35 in astrocytes was increased during early
phases and decreased during late phases after OGD/R. Conclusion: Both Cdk5 and p35 were expressed in
astrocytes, and both showed similar, mainly cytoplasmic distribution. Cdk5 and p35 expression in astrocytes
exhibited dynamic changes after OGD/R. |
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