| 李颖慧
，李军
，任思颖
，王丽琨
，伍国锋.罗格列酮对家兔脑出血模型病灶周围内皮素-1表达
及血脑屏障通透性的影响[J].神经损伤功能重建,2018,13(3):109-112 |
| 罗格列酮对家兔脑出血模型病灶周围内皮素-1表达
及血脑屏障通透性的影响 |
| Rosiglitazone Influences Expression of ET-1 and Permeability of Blood Brain Barrier inRabbit Model of Cerebral Cemorrhage |
| |
| DOI： |
| 中文关键词: 脑出血 内皮素-1 血脑屏障通透性 罗格列酮 |
| 英文关键词: cerebral hemorrhage ET-1 blood-brain barrier permeability rosiglitazone |
| 基金项目:国家自然科学基金
（No. 81460185）；
贵州省科学技术基
金[No. (2013)2043]；
贵州省高血压性脑
出血微创诊疗科技
创新人才团队基金
[黔科合人才团队
(2014)2040]；
贵阳市科技计划项
（ 筑 科 合 同
[20161001]51 号 及
[2017]5-1号） |
|
| 摘要点击次数: 3588 |
| 全文下载次数: 3746 |
| 中文摘要: |
| 目的：探讨罗格列酮对家兔脑出血（ICH）模型血肿周围脑组织内皮素1（ET-1）表达及血脑屏障通透性
的影响。方法：45只健康家兔随机分为正常对照组（NC组）、模型对照组（MC组）及罗格列酮灌注组（RSG
组），各15只。自体血注入法制备基底核区ICH模型。造模后6 h，RSG组给予罗格列酮病灶区灌注，各组分
别在处理后1、3和7 d处死，每时间点5只。处死前行Purdy神经功能评分，伊文思蓝法判断血脑屏障通透
性，比较血肿周围脑组织ET-1表达，分析ET-1表达水平与血肿周围脑组织伊文思蓝含量及Purdy神经功能
评分相关性。结果：与NC组比较，MC组和RSG组出现显著神经功能损害（P<0.05），病灶周围ET-1表达、血
脑屏障通透性均显著增加（P<0.05）；与MC组比较，RSG组神经功能损害减轻，病灶周围ET-1表达、血脑屏
障通透性均降低（P<0.05）；血肿周围脑组织ET-1表达水平与血脑屏障通透性及Purdy神经功能评分呈显著
相关性（P<0.01）。结论：ICH后血肿周围脑组织ET-1表达及血脑屏障通透性增加，罗格列酮灌注治疗可降
低血肿周围脑组织ET-1表达及血脑屏障通透性，减轻神经功能损害。 |
| 英文摘要: |
| To investigate the effect of rosiglitazone on perihematomal brain tissue endothelin-1
(ET-1) expression and blood-brain barrier permeability in rabbits with intracerebral hemorrhage (ICH).
Methods: Forty-five healthy rabbits were randomly divided into a normal control group (NC group), model
control group (MC group), and rosiglitazone perfusion group (RSG group), with 15 rabbits per group.
Autologous blood injection was used to establish basal ganglia ICH model. Six hours after generating model,
rabbits in the RSG group were given a rosiglitazone perfusion to the lesion site. Each group was divided into
three subgroups (5 rabbits per subgroup), and animals in each subgroup were euthanized on days 1, 3, and 7
post-procedure. Before euthanasia, neurological deficit scores (Purdy scores) were determined and Evans blue
staining was performed to determine blood-brain barrier permeability. After rabbits were sacrificed,
perihematomal brain tissue was removed to determine ET-1 expression. Data was analyzed for correlation
between ET-1 expression levels and Evans blue stained perihematomal brain tissue and Purdy scores. Results:
In MC and RSG groups, significant neurological impairment was found (P<0.05), and ET-1 expression and BBB
permeability surrounding the lesion site were significantly increased compared to the NC group (P<0.05). When
compared to the MC group, the RSG group showed a decrease in neurological impairment, ET-1 expression
surrounding the lesion, and BBB permeability (P<0.05). ET-1 expression in perihematomal brain tissue showed
significant correlation with blood-brain barrier permeability and Purdy neurological deficit scores (P<0.01).
Conclusion: ET-1 expression in perihematomal brain tissue and blood-brain barrier permeability increased after
intracerebral hemorrhage. RSG infusion therapy can reduce the expression of ET-1 in brain tissue around
hematomas and decrease the permeability of the blood-brain barrier, thereby reducing neurological damage. |
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