文章摘要
白超博，佡剑非.Hoehn-Yahr分级为1期的帕金森病患者外周血 基因差异表达分析[J].神经损伤功能重建,2018,13(2):69-72
Hoehn-Yahr分级为1期的帕金森病患者外周血 基因差异表达分析
Gene Expression Profiles Analysis of Peripheral Blood in Stage 1 PD Patients (Hoehn–YahrScale)
  
DOI：
中文关键词: 帕金森病  Hoehn-Yahr分级  差异表达基因  通路富集分析
英文关键词: Parkinson disease  Hoehn–Yahr scale  differentially expressed gene  pathway enrichment analysis
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作者单位
白超博，佡剑非 中国医科大学附属 盛京医院第三神经 内科 
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中文摘要:
      目的：对Hoehn-Yahr分级为1期的帕金森病（PD）患者外周血进行基因芯片研究，分析PD患者外周血 基因表达的变化。方法：从 GEO 数据库中获得 Hoehn-Yahr 分级为 1 期的 PD 患者外周血基因数据集 GSE54536。运用GEO2R筛选差异表达基因，DAVID工具进行富集基因功能和通路分析。STRING数据库 构建差异表达基因蛋白相互作用的网络，应用CytoScape软件筛选核心基因。结果：共筛选到997个差异基 因，涉及细胞间信号转导、细胞分泌等生物学过程及PI3K-Akt等信号通路。c-fos、KDM6B、ESR1等为核心 基因。结论：通过对Hoehn-Yahr分级为1期的PD患者外周血的基因芯片进行研究，提示PD为多种基因，多 种分子机制相互作用的结果，对相关分子机制的研究有助于进一步阐明PD患者的发病机制。
英文摘要:
      To investigate gene expression profiles of peripheral blood in patients with Hoehn– Yahr scale stage 1 Parkinson’s Disease (PD) via microarray analysis. Patient peripheral blood was studied for variations in gene expression. Methods: Microarray dataset GSE54536 containing peripheral blood genetic data of stage 1 PD patients was downloaded from the Gene Expression Omnibus (GEO) database. The data was analyzed by bioinformatics methods using GEO2R for screening of differentially expressed genes and DAVID for gene pathway enrichment analysis. Based on these genes, STRING database was used to construct protein interaction networks, and the data obtained was used by CytoScape to screen for hub genes. Results: A total of 997 genes were identified as differentially expressed genes. The main biological pathways involved included cell-cell signaling, cell secretion, and PI3K-Akt signaling pathways. The hub genes included c-fos，KDM6B，and ESR1. Conclusion: The data from microarray analysis of peripheral blood of stage 1 PD patients suggest that PD involves multiple genes and multiple molecular mechanisms. Investigation of these genes may provide valuable insights into the mechanism of PD development in patients.
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