文章摘要
冯楚文 ，鲁菁 ，屈媛媛 ，孙维伯 ，刘婷婷 ，吴永芳 ，杨添淞.通督御疲调神电针调节线粒体功能改善慢性疲劳综合征认知障碍的机制研究[J].神经损伤功能重建,2025,(知网首发):
通督御疲调神电针调节线粒体功能改善慢性疲劳综合征认知障碍的机制研究
Study on the Mechanism of Tongdu Yupi Tiaoshen Electric Acupuncture ImprovingCognitive Impairment in Chronic Fatigue Syndrome by Regulating Mitochondrial Function
  
DOI：
中文关键词: 慢性疲劳综合征  认知障碍  线粒体分裂  线粒体自噬  电针
英文关键词: chronic fatigue syndrome  cognitive impairment  mitochondrial division  mitochondrial autophagy  electroacupuncture
基金项目:黑龙江省自然科学基金 （电针调控线粒体动力 学促进 CFS 认知障碍 大鼠突触重塑的机制研 究，No. LH2024H050）； 国家自然科学基金（电 针调控 MDM2 泛素化 PSD-95 水平重塑海马 神经元突触结构改善 CFS认知功能障碍的机 制研究，No.82074539； 艾灸激活CaMKⅡ磷酸 化介导长时程增强调节 突触可塑性治疗 CFS 认知障碍的机制研究， No.82305394）；教育部 “春晖计划”合作科研项 目（“通督御疲”调神针 法改善 CFS 认知功能 障碍海马突触可塑性的 效应机制研究，No. HZ KY20220308-20220135 7）；黑龙江省中医药科 研课题（电针调节 CFS 认知功能障碍大鼠海马 突触可塑性的作用机制 研究，No. ZHY2022-13 6）；黑龙江省中医药学 会青年人才托举工程项 目（“通督御疲调神针 法”改善 CFS 认知功能 障碍海马突触可塑性的 效应机制研究，No. 202 2-QNRC1-05）；黑龙江 省青年岐黄学者培养项 目（黑中医药教函[2023] 36号）。
作者单位
冯楚文1,2 ，鲁菁3 ，屈媛媛1,2 ，孙维伯4 ，刘婷婷3 ，吴永芳3 ，杨添淞1,2 1. 黑龙江中医药大学附 属第一医院康复二科 2. 黑龙江省中医药信息 学重点实验室 3. 黑龙江中医药大学研 究生学院 4. 哈尔滨医科大学研究 生学院 
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中文摘要:
      目的：本研究旨在探讨通督御疲调神电针对慢性疲劳综合征（chronic fatigue syndrome，CFS）认知 障碍大鼠的疗效及对大鼠海马CA1区线粒体分裂及线粒体自噬的影响。方法：采用慢性多因素复合应 激刺激法制备CFS认知障碍大鼠模型。将造模成功的大鼠随机分为模型组和电针组各10只，10只健康 雄性SD大鼠为对照组。治疗期间，对照组正常饲养，模型组持续造模刺激，电针组在造模刺激基础上予 神庭穴透刺百会穴、大椎穴电针治疗；参数设置为：连续波，频率10 Hz，15 min/次/d，连续28 d。采用《一 般情况半定量观察量表》及Morris水迷宫评价各组大鼠一般情况及学习、记忆能力；透射电镜观察各组 大鼠海马CA1区线粒体超微结构；Western Blot及RT-qPCR检测各组大鼠海马CA1区线粒体自噬及线 粒体分裂关键分子Pink1、Parkin及Drp1的蛋白和mRNA表达水平。结果：治疗后，与对照组比较，模型 组一般情况评分升高（P＜0.05），逃避潜伏期延长（P＜0.05），穿越平台次数减少（P＜0.05）；海马CA1区 线粒体破坏增加，形态呈肿胀或缩小，基质变淡且伴空泡化，嵴突消失，线粒体外膜不完整，出现自噬小 体；Pink1、Parkin、Drp1蛋白及mRNA表达水平下降（P＜0.05）。与模型组比较，电针组一般情况评分降 低（P＜0.05），逃避潜伏期缩短（P＜0.05），穿越平台次数增多（P＜0.05）；海马CA1区线粒体破坏数量及 自噬小体数量较少，形态呈轻度肿胀，内嵴较清晰，嵴有断裂但嵴突未消失，外膜大致完整，基质改变不 显著；Pink1、Parkin、Drp1 蛋白及 mRNA 表达水平上调（P＜0.05）。结论：通督御疲调神电针具有抗疲 劳，提高认知水平的作用，可能与调节CFS认知障碍大鼠线粒体自噬及线粒体分裂功能有关。
英文摘要:
      This study aimed to explore the efficacy of Tongdu Yupi Tiaoshen electric acupuncture on cognitive impairment in rats with chronic fatigue syndrome (CFS) and its impact on mitochondrial fission and mitophagy in the hippocampal CA1 region. Methods: A CFS cognitive impairment rat model was established using a chronic fatigue multifactorial compound stress method. The successfully modeled rats were randomly divided into a model group and an electroacupuncture group, with 10 rats in each, while another 10 healthy male SD rats served as the control group. During the treatment period, the control group was fed normally, the model group continued to receive modeling stimulation, and the electroacupuncture group received additional electroacupuncture treatment at the GV24 puncture GV20 and the GV14, with a continuous wave, frequency 10 Hz, 15 minutes per session per day, for a consecutive 28 days. The general condition and learning and memory abilities of rats in each group were evaluated using the General Conditions Semi-Quantitative Observation Scale and Morris water maze; transmission electron microscopy was used to observe the ultrastructure of mitochondria in the hippocampal CA1 region of rats in each group; Western Blot and RT-qPCR were used to detect the protein and mRNA expression levels of key molecules Pink1, Parkin, and Drp1 related to mitochondrial autophagy and mitochondrial fission in the hippocampal CA1 region of rats in each group. Results: After treatment, compared with the control group, the general condition score of the model group was increased (P<0.05), the escape latency was prolonged (P< 0.05), and the number of platform crossings was reduced (P<0.05); mitochondrial damage in the hippocampal CA1 region was increased, with swelling or shrinking in shape, lightened matrix with vacuolization, disappearance of cristae, incomplete outer membrane, and the appearance of autophagic bodies; the protein and mRNA expression levels of Pink1, Parkin, and Drp1 were decreased (P<0.05). After treatment, compared with the model group, the general condition score of the electroacupuncture group was decreased (P<0.05), the escape latency was shortened (P<0.05), and the number of platform crossings was increased (P<0.05); there were fewer damaged mitochondria and autophagic bodies in the hippocampal CA1 region, with mildly swollen shape, relatively clear inner cristae, cristae fracture but no disappearance of cristae protrusions, roughly intact outer membrane, and no significant change in matrix; the protein and mRNA expression levels of Pink1, Parkin, and Drp1 were upregulated (P<0.05). Conclusion: Tongdu Yupi Tiaoshen electric acupuncture has anti-fatigue and cognitive-improving effects, which may be related to the regulation of mitochondrial autophagy and mitochondrial fission function in CFS cognitive impairment rats.
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