| 孟宪宇
，李秋红
，刘岩
，张旭
，王磊
，姜巍
，李凤久.碳量子点（CDs）耦合Annexin-V抗体复合物活体SD大鼠周围神经功能束鉴别的基础研究[J].神经损伤功能重建,2024,(知网首发): |
| 碳量子点（CDs）耦合Annexin-V抗体复合物活体SD大鼠周围神经功能束鉴别的基础研究 |
| Identification of Peripheral Nerve Functional Fascicles in Sprague-Dawley (SD) Rats byCarbon Quantum Dots (CDs) Coupled with an Annexin-V Antibody Complex |
| |
| DOI： |
| 中文关键词: 周围神经 感觉束与运动束 鉴别 Annexin-V 碳量子点 |
| 英文关键词: peripheral nerve sensory fascicles and motor fascicles distinguish Annexin-V carbon
quantum dots |
| 基金项目:黑龙江省自然科学基
金联合引导项目（碳
量 子 点（CDs）偶 联
Annexin-V 抗体复合
物活体Sprague-Dawley大鼠周围神经功能
束鉴别的基础研究，
No. LH2019H050）；
黑龙江省中医药科研
项目（自研一体化夹
板规范化治疗桡骨远
端骨折的临床研究，
No. ZHY19-032）；黑
龙江中医药大学教育
教学研究基金项目
（基于疾速原型制造
技术的临床实践教学
法在中医骨伤科课程
中 的 实 施 与 探 索 ，
No. XJJ2014006）；高
等学校博士学科点专
项 科 研 基 金（应 用
Annexin V 抗体偶联
CdTe 纳米量子点进
行周围神经感觉与运
动束鉴别的基础研
究，No. 20132327120
004）；哈尔滨市科学
技术局科技计划项目
（3D关节成形垫片微
型外固定组合架治疗
手部指间关节粉碎性
骨折（Pilon 骨折）的
应用研究，No. 2022Z
CZJNS057） |
|
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| 中文摘要: |
| 目的：探寻术中可用的周围神经功能束鉴别的方法。方法：分组制备SD大鼠双侧股神经主干、股
神经肌支及隐神经Sunderland V损伤模型，制备的Annexin V-碳量子点（Carbon quantum dots，CDs）抗体
复合物涂抹神经断端，在380 nm紫外灯激发光源照射下，5、10、15和20 min后在荧光显微镜下观察荧光
显色。 结果：SD大鼠双侧股神经主干、股神经肌支及隐神经Sunderland V损伤断端染色5 min荧光强度
不足以充分显示神经束；染色 10～15 min 强度明显增加，股神经主干的感觉束及隐神经在 Annexin
V-CDs抗体复合物染色下呈蓝色荧光，股神经主干的运动束及股神经肌支无荧光显示；染色20 min后荧
光强度逐渐减弱。各分组组内样本染色5、10、15、20 min的染色强度无明显差异。结论：Annexin V-CDs
抗体复合物能够实现术中SD大鼠周围神经功能束的鉴别。 |
| 英文摘要: |
| To explore a method to rapidly differentiate between the sensory and motor fascicles
in peripheral nerves for clinical application. Methods: The Sunderland V injury model was induced in the
bilateral femoral nerve trunk, the muscle branch of the femoral nerve and the saphenous nerve of
Sprague-Dawley (SD) rats. The Annexin V-Carbon quantum dots (CDs) antibody complex was prepared and
applied to the severed nerve ends. Fluorescence was assessed under a 380 nm UV light source at 5, 10, 15 and
20 minutes after staining under a fluorescence microscope. Results: The fluorescence intensity at 5 minutes
was insufficient to fully display nerve bundles in the injured bilateral femoral nerve trunk, the muscle branch
of the femoral nerve, and the saphenous nerve of SD rats. However, the intensity of staining increased
significantly between 10 to 15 minutes, with the sensory fascicles of the femoral nerve trunk and saphenous
nerve showing blue fluorescence upon Annexin V-CDs antibody complex staining, while no fluorescence was
observed in the motor fascicles of the femoral nerve trunk or the muscle branch of the femoral nerve. The
fluorescence intensity gradually decreased after 20 minutes. There were no significant differences in the
staining intensity within each group at 5, 10, 15 or 20 minutes. Conclusion: The Annexin V-CDs antibody
complex can be used to effectively distinguish sensory from motor fascicles in peripheral nerves
intraoperatively in SD rats. |
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